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battery The new photovoltaic power plant dust coating technology to solve proble

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In our solar p battery hotovoltaic project because the coating small dust can escape led to the loss of several hundred million dollars annually. This is not alarm battery ist, but a fact! It is understood that China's solar power plant due to contamination by the dust, resulting in decreased efficiency of solar panels, the

Eye canal insights and importance for glaucoma

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New insights into a specialised eye structure called the Schlemm’s canal may be critical in advancing research on the blinding eye disease glaucoma, which affects over 70 million people globally. The insights are presented in a new study from a research team based in Jackson Laboratory and in Tufts University School of Medicine in Boston, published today in the journal PLoS Biology.

Schlemm’s canal, essentially a tube lined with endothelial cells, is vital for control of eye fluid flow and of intraocular pressure. It is directly implicated in glaucoma. However, its role is poorly understood. In the current study, the researchers used mouse models engineered to express fluorescent proteins and developed a ‘whole mount’, three-dimensional approach to the study of these animals. Using this novel methodology, they were able to observe how the Schlemm’s canal forms and both in regard to the eye and to adjacent tissues.

As a result of the study, the researchers identified a unique vascular development process they called ‘canalogenesis’ which combines elements of angiogenesis, vasculogenesis and lymphangiogenesis but also novel features that distinguishes it from all of them. They also identified a vascular endothelial growth factor receptor, a receptor tyrosine kinase called kinase insert domain receptor, as functionally important in early Schlemm’s canal development.

Another important finding was that the endothelial cells (SECs) lining the Schlemm’s canal were shown to have properties of blood and lymphatic endothelial cells. First author Dr Krishnakumar Kizhatil, an associate research scientist in the laboratory of JAX Professor and Howard Hughes Medical Investigator Dr Simon John explains the significance of this finding “Thus, Schlemm’s canal is a unique vessel with endothelial cells that are highly specialized for its complex functions…This resolves a long-standing controversy about the cellular phenotype of SECs.”

Co-author Dr Jeffrey Marchant of Tufts concludes: “This study lays a critical new foundation for determining the functions of Schlemm’s canal both in maintaining ocular health and when things go wrong in glaucoma.”

News source: PLOS Biology

News reference: Kizhatil K, Ryan M, Marchant JK, Henrich S, John SWM (2014) Schlemm’s Canal Is a Unique Vessel with a Combination of Blood Vascular and Lymphatic Phenotypes that Forms by a Novel Developmental Process. PLoS Biol 12(7): e1001912. doi:10.1371/journal.pbio.1001912; available at: http://www.plosbiology.org/article/info:...io.1001912

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Enrollment in genetics undergraduate degree

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Hi all!

I'm a freshman currently enrolled at a university, and am pursuing a genetics major. Problem is, I would like to pursue genetic engineering as a career - will I be able to go to graduate school, studying genetic engineering, with my current genetics degree?

The research I have done online suggests that I should take a biomedical engineering undergraduate course, instead of a genetics course. What would be the best solution to this?

Please give me your thoughts and opinions on this, thank you!

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Visualising the architecture of G-protein coupled receptor interactions

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The architecture of the key regulatory protein beta-arrestin in complex with a G-protein coupled receptor (GPCR) has been elucidated by a combination of electron microscopy and mass spectrometry in a paper published in the journal Nature. The study comes from a research team from Duke Medicine, the University of Michigan and Stanford University. The study should help advance understanding of how cells transmit signals and how signalling is controlled in the body's response to stimuli including light and pain.

GPCRs are seven transmembrane spanning structures which operate in a multitude of cell signalling pathways. As a group, they represent the largest drug target family for human diseases as disparate as cardiovascular disease, neurological disease and cancer. Co –senior author on the paper, Dr Robert J. Lefkowitz of Duke University School of Medicine and the Howard Hughes Medical Institute, explains the importance of insights into the structure of GPCRs: "It is crucial to visualize how these receptors work to fully appreciate how our bodies respond to a wide array of stimuli, including light, hormones and various chemicals." Dr Lefkowitz shared the Nobel Prize for Chemistry in 2012 with one of the other senior co-authors on the paper, Dr Brian K. Kobilka, of Stanford University School of Medicine, for their work on GPCRs.

In this paper, the authors presented a visualisation of the protein beta-arrestin in complex with the ‘fight-or-flight’ associated beta 2-adrenergic receptor. Beta-arrestins function to desensitise and therefore cap GPCR signalling and to initiate a new wave of GPCR-independent signalling. Another co-senior author, Dr Georgios Skiniotis of the University of Michigan further explains: "Arrestin's primary role is to put the cap on GPCR signaling. Elucidating the structure of this complex is crucial for understanding how the receptors are desensitized in order to prevent aberrant signalling." The research team formed and purified a functional human β2AR–β-arrestin-1 complex. Using mass spectrometry, cross-linking analysis and electron microscopy, they were able to confirm a previously suspected but not demonstrated bimodal binding, involving two separate interactions between beta-arrestin and both the intracellular carboxy tail of the GPCR and with its 7 transmembrane core.

The authors now plan to bring X-ray crystallography into play in order to attain atomic level insights into this architecture. These details could then be harnessed in experiments aimed at novel drug design and for gaining improved understanding of the fundamentals of GPCR biology. Co-lead author Arun K. Shukla concludes: “This is just a start and there is a long way to go…We have to visualize similar complexes of other GPCRs to develop a comprehensive understanding of this family of receptors."

Sources

Shukla, A.K. et al (2014). Visualization of arrestin recruitment by a G-protein-coupled receptor. Nature (June 22 2014); doi:10.1038/nature13430

Press release: Duke University Medical Center; available from http://www.eurekalert.org/pub_releases/2...061914.php

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1.0 DNA BAR CODING

1.1 INTRODUCTION.
DNA barcode is a unique pattern of DNA sequence that identifies each living thing using short DNA barcodes about 700 nucleotides or more in length, can be quickly processed from thousands of specimens and then analyzed by computer programs.

It’s a type of taxonomic method that uses a short genetic marker in an organism’s DNA to identify it as belonging to a particular species. It came first to the attention of the scientific community in 2003 when a group of scientific researchers of the university of Guelph published a paper titled “Biological identification through DNA barcodes”. In it, they proposed a new system of species identification and discovery using a short section of DNA from a standardized region of the genome. The gene region that is being used for almost all animals is 648 base-pair region of the mitochondrial cytochrome c oxidase subunit I gene (COI), which is proving highly effective in identifying birds, butterflies, fish, flies and many other animal groups.

The choice of taking the mitochondrial gene region COI is because all eukaryote cells contain mitochondrial containing mitochondrial DNA (mtDNA) which it maternally inherited and has a high mutating rate which results in significant variation in mtDNA sequences between species and brings a small variation in animals. A 648-bp region ( the folmer region) of the mitochondrial cytochrome c oxidase subunit I, was proposed as a potential barcode.

1.2 USE OF DNA BARCODES.
The major use of DNA barcode is classifying different animal species at a molecular level, use of molecular biology techniques such as PCR and Gene sequencing to identify and classify organisms based on short sequences of their genes that are specific and unique only to that specific species.

1.3 DNA BARCODE FORMATION, IDENTIFICATION AND STORAGE.
DNA barcodes are created following a number of steps starting from obtaining cell samples from a certain animal species, perform DNA extraction, run polymerase chain reaction (PCR) so as to amplify a certain region of interest of the DNA then run DNA sequencing so as to identify the region. The DNA region can be compared with a control sequence in a data base so as to identify the particular species or to update the data base with the new DNA sequence of a new species.

A standardized library of barcodes will enable more people i.e experts and non-experts to identify species whether abundant or in a rare case, with recent development of technology DNA barcode are preserved in large data bases such as the Barcode of life Data Systems (BOLD) at the university of Guelph, Canada. Today more over 290,000 records have been banked, representing over 31,000 species and data accumulate at an accelerating rate, this gives easy access to scientists and non-scientists all over the world to update and process different DNA sequences of different species.

1.4 SIGNIFICANCES OF DNA BARCODE.
Identifying organisms has grown in importance as we monitor the biological effects of global climate change and attempt preserve species diversity in the face of accelerating habitat destruction. Now DNA barcodes allow non-experts to objectively identify species even from small, damaged, or industrially processed materials, as the unique pattern of bars in a universal product code (UPC). This helps in preserving them, monitoring their development and stopping them from getting extinct.

Particular in forensic sciences on the part of the crime laboratory investigation, DNA barcode acts as not only the new technology of identifying specific species but the key to solving criminal cases involving poaching of different animals but mostly the endangered ones and the wildlife industry in general, by linking the victims (animals) and suspects makes it easier for the prosecutor to solve these cases. This system has helped a lot in developed countries in solving cases but it has being a problem in developed countries due to the lack of appropriate technology and poor storage of DNA barcodes of the species.

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JBEI GT collection is a new biofuels research resource

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Efforts to advance production of environmentally friendly transportation fuels from plant biomass should be aided by a new glycosyltransferase clone collection compiled by researchers at the U.S. Department of Energy (DOE)’s Joint BioEnergy Institute (JBEI). A paper on the collection has been accepted for publication in The Plant Journal. The aim of the JBEI GT collection is to provide a functional genomic for researchers in the field of sugar extraction from plant biomass to product fuels.

Glycosyltransferases (GT) are the enzymes responsible for catalysis of simple plant monosaccharides into complex polysaccharides needed for many plant cells function and structures. Large GT families have evolved in plants but their chemical nature has hampered efforts to understand specific functions of the majority of GTs. This hinders bioenergy research efforts to modify plant biomass with a goal to produce maximum amounts of transportation fuels.

In an attempt to address this issue, the JBEI team, led by Dr Joshua Heazlewood, director of the JBEI Plant Systems Biology Programme, have cloned and verified the new clone collection which contains 403 Arabidopsis GTs (the reference plant for poplar and other similar species) and 96 rice GTs (the reference plant for grasses). This represents about 88% of the known Arabidopsis GTs but only about 15% of rice GTs, so the team aim to extend this. Dr Heazlewood explains the ethos and utility behind the clone collection: “Using the unique infrastructure and resources at JBEI, we have provided a collection of high quality GT clones, all of which have been verified by sequencing and are available in easy to use cassettes…We’re making this entire collection available to the plant research community and expect it to drive our basic understanding of GTs and enable the manipulation of cell walls.”

As well as the GT clone collection, the team have produced a set of so-called ‘pBullets’ which are particle bombardment plasmids that mark targeted protein location with high efficiency when shot into cells. The JBEI pBullets are designed to mark elements of the plant endomembrane system, which separates the functional and structural plant cell components. Dr Heazlewood explains how this system could be useful: “Our pBullet vector series is custom designed for efficient bombardment…Researchers generally use large unwieldy plasmids that perform badly when it comes to localizing proteins.”
More information on the JBEI GT collection and the pBullets can be found on the JBEI Website at http://gt.jbei.org/

Sources

Lao, J. et al. (2014). The Plant Glycosyltransferase Clone Collection for Functional Genomics. The Plant Journal (accepted article); DOI: 10.1111/tpj.12577

Press release: The Berkeley Lab; available from https://newscenter.lbl.gov/2014/06/23/th...ollection/ [Accessed 24 June 2014].

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6 months internship Training program on Clinical research & Data Management, PV
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45 Days Training Program on SAS Clinical Programming

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2 industrial projects will be assessed every individual person
Flexible Lab facilities
Mock Interviews
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Soft skills development
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Note: Hands-On practical knowledge on /Database Programming –Inform Architecture/Central Designer)

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B.Tech (CSE, IT)

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