I am currently performing a salmonella mutagenicity assay and experiencing some difficulty.
I am culturing a frozen stock into nutrient broth and growing a 16 hour culture and there is growth in my broth. When culture is transferred to top agar and plated on minimal glucose base there is no growth of culture.
Does anyone have any suggestions?
Thank you!
I am culturing a frozen stock into nutrient broth and growing a 16 hour culture and there is growth in my broth. When culture is transferred to top agar and plated on minimal glucose base there is no growth of culture.
Does anyone have any suggestions?
Thank you!