Hi
I am Ph.D scholar of Biotechnology doing research on expression of mutated gene. I have to use blunt and sticky ligation in same reaction by using thermo scientific fast digest enzymes and ligase . Any one use this strategy ?
I am Ph.D scholar of Biotechnology doing research on expression of mutated gene. I have to use blunt and sticky ligation in same reaction by using thermo scientific fast digest enzymes and ligase . Any one use this strategy ?