need guidance for my career improvement

hi all
i am arti kumari in biotechnology in 2005. after that due to family reason i was not able to join any of the research institute or industry. in april 2008 i joined as research assistant at rajendra memorial research institute of medical sciences, at patna bihar. i worked till april 2010. after that i am not able to join any where for my further career . how can i overcome this gap in my career. please anyone suggest me. i want to do phd in biotechnology.
please anyone suggest me.