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Scientists Succeeded to Produce Electricity Using Viruses

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First step towards the development of tiny devices that produce electricity while performing everyday tasks is new approach. Imagine that you charge your cell phone while walking, thanks to the power generator thin like paper embedded in your shoe. This futuristic scenario is now a little closer to reality. Scientists from Lawrence Berkeley National Laboratory (Berkeley Lab), developed a way to produce electricity using harmless viruses that convert mechanical energy into electrical energy.

Researchers tested their methodology of creating a generator that produces enough electricity to run small liquid-crystal displays. It activates by touching the electrode size of a postage stamp. The electrode is coated with a layer of the viruses created specifically for this purpose. Viruses convert force of the touch into the electrical discharge.

Piezoelectricity

This is the first power generator that uses piezoelectric properties of biological materials. Piezoelectricity is the accumulation of charge in a solid material as a response to mechanical stress.

This process can lead to the production of small devices that generate electrical energy from the vibrations that occur when performing everyday tasks such as closing doors or climbing stairs. It also indicates the way to make microelectronic devices more easily. This is possible because the viruses organize themselves in a film that allows the operation of this generator. This is great advantage of manipulation with viruses.

"Further research is needed, but our work is a promising first step towards the development of personal generators for electricity, starters for use in nano-devices and other devices based on viral electronics," said Seung-Wuk Lee, a scientist at Lawrence Berkeley National Laboratory and an associate professor of bioengineering at the University of California, Berkeley. He conducted the study with a team that included Ramamoorthy Ramesh and Lee Byung Yang.

The piezoelectric effect was discovered in the 1880th and since then, it was observed in crystals, ceramics, costumes, proteins and DNA. It is also used a lot in practice. Electric igniters and microscopic scanners could not exist without it, just to mention a few of its applications. However, the materials used for making piezoelectric devices are highly toxic and difficult to work with, which limits the widespread use of this technology.

Bacteriophage M13

Lee and his colleagues wondered whether the virus is being studied in laboratories around the world to offer a better way of solving these problems. Bacteriophage M13 attacks only bacteria, while it is harmless to humans. Since it is a virus, it replicates itself in millions of copies in a few hours, so there is always a constant amount of virus available. It is also very easy to genetically modify the virus. In addition, a large number of such viruses naturally orient themselves in a beautifully decorated films.

These are the properties that scientists are looking for in order to make basic unit in nano technology. However, the researchers first had to determine whether the virus M13 is piezoelectric. Lee and Ramesh, who is an expert in the study of electrical properties of thin films in nano technology, applied the electric field to the film of the virus M13 and watched using special microscope what was happening. Helical proteins that cover viruses were inverted and opened in response to an electric field, which is a sure sign of the piezoelectric effect in action.

Furthermore, scientists have increased the piezoelectric effect of the virus. They have added to viruses four negatively charged amino acid residues at one end of the coil proteins that cover the virus. These residues increased the charge difference between the positive and the negative end of the protein, and that raised the voltage of the virus.

Scientists have further enhanced the system of film composition made of individual layers of the virus. They found that the film composed of 20 single layers showed the strongest piezoelectric effect.

The First Generator

What had left was to make a test. Therefore, scientists have created a piezoelectric power generator based on the virus. Such conditions are created for genetically modified viruses to be spontaneously organized in those multi-layered films that had the size one square centimeter. After that, the film is placed between two golden electrodes that are wired to a liquid crystal display. When pressed on the generator, the electricity of 6 nA and 400 millivolts is produced. That's enough electricity to turn up the number "1" on the screen.

"Now we are working on improvement the results of this demonstration which was supposed to show the correctness of our way of thinking," says Lee. "Because the tools of biotechnology allow mass production of genetically modified viruses, piezoelectric materials based on viruses could offer a simple way to develop new microelectronic devices in the future."

Creation of Artificial Cells

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Creation of chain DNA in laboratories leads to the creation of artificial organisms. Scientists are now able to make a DNA chains "on demand", such as they need. There are numerous reviews on this revolutionary scientific discovery, because many have fear of the outcome. Like the experts say: "you should not be playing God."

After 15 year-work, a team of U.S. researchers has developed the first auto reproductive living cell, which is controlled by a synthetic genome. Critics warn of the risk of developing artificial organisms. "It is a cell that is derived from fully synthetic chromosome, made of four bottles of chemicals that make up DNA parts," said Craig Venter, chief researcher at the Institute Jay Craig. Venter told reporters that the experiment began with a live cell, which is then processed using a synthesized genome, after which the cells pass through a "million steps of replication." The researchers first constructed a kind of "genetic software" bacteria, which were then transplanted into the cell, and eventually frozen. Otherwise, Venter said that two years ago, managed to make sensitization of bacterial genome, but had not been able to transplant it into a cell.

Except synthesizing the genome (the complete hereditary basis of an organism) by fusing the chromosomes which contain the DNA of the organism, the genome researchers added "watermarks" to distinguish them from natural. These tags, in fact, contain the names of all 46 authors and researchers who worked on the project, as well as the official website, so that anyone who succeed to perform decoding can send e-mail to the team. In addition, three quotes of Irish writer James Joyce are included. One of them sounds as follows: "To live, to be mistaken, to win, to create life out of life." With good reason, apparently. Venter added that "this is the first time that a synthetic DNA is in full control of a single cell." That is why he will continue to work on the construction of the bacteria to be able to perform useful functions.

Algae from glass bottles AGAINST "greenhouse"

This method could be used in the manufacture of medicines, vaccines and biofuel, the new, so-called "clean energy" for the refinery. It also, could enable the production of algae for absorption and clearance of carbon dioxide, the most dangerous environmental pollutant, according to the report published in the journal "Science." "We are on course to develop a very powerful apparatus in an attempt to control biological processes," said Craig Venter, researcher who was involved in mapping the human genome in the last 10 years. During that research, he determined the location of each human gene and its specific structure and function.

Opening Pandora's Box

Critics warn that Venter and his colleagues that way entered in the danger zone, because "they have created artificial organisms that have not been fully studied and understood." Venter stood in front of the public, and, in his defense, he said that he "wasn’t trying to play God." "It's a classic charge that critics point when scientists discover something new in medicine or biology. Man has always lived in attempt to control nature. Didn’t that cause creating the domestic animals," said the researcher. He believes that this is not an act of opening a Pandora's box, but on the contrary - "This is a step, a small step forward in realizing and understanding the nature and functioning of life, and perhaps, in discovering ways to control microbial world, to improve people's lives."

This experiment raised many ethical questions. Pat Mooney, director of ETC Group in Canada, said that the creation of artificial cells "is not SOS shop for all our troubles in the society. It is more likely that this will create a series of new problems." Among them, according to the possibility of abuse, primarily belong weapons for bioterrorism. Rejecting the possibility, Winter concluded that "technology and the cells are not for sale”.

Strive to use technology in order to improve the quality of human life, as well as to improve knowledge and understanding of cellular life is spreading with great speed. Although there are different opinions whether this kind of technology should be applied, the progress of science seems unstoppable. We can only imagine the incredible advantages that future generations are going to experience thanks to biotechnology achievements.

Can rh negative blood be cloned?

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Hi everyone,
I run a community of rh negative people and the claim that rh negative blood cannot be cloned keeps popping up. I would like to know from some of you if
1) this is correct
and
2) why.

Best,

Mike Dammann

Scientific Sensation: Paralyzed Patients Will Walk Again

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Rats with spinal cord injury and severe paralysis are now walking (and running) thanks to researchers at the Federal Polytechnic School of Lausanne (EPFL). The findings published in June 2012 in the Science journal, show that interrupted spinal cord can regain its function when it awakens its own intelligence and ability to regenerate. The research, which began five years ago at the University of Zurich, indicated profound changes in our understanding of the central nervous system. It is not yet clear whether similar techniques and rehabilitation would work in humans, but there is nerve growth that suggests new methods for treating paralysis.

Neurorehabilitation

"After a few weeks of neurorehabilitation with a combination of robotic armor and electrochemical stimulation, our rats not only moved their legs, but also ran, climbed the stairs and avoided obstacles”, said Courtin, who holds the Chair of recovery of the spinal cord at the EPFL.

Neuroplasticity

It is well known that the brain and spinal cord can adapt and recover from moderate injuries, and this is a quality known as neuroplasticity. So far, the spinal cord showed so little neuroplasticity following severe injuries from which the recovery was impossible. Courtin’s research shows that, under certain conditions, plasticity and recovery can occur even in these difficult cases, but only if the dormant spinal cord in the spine first wakes up.

Waking Up The Spinal Cord

To do this, Courtine and his team injected in the laboratory rat a chemical solution of monoamine agonists. These chemicals trigger responses of the cells by binding to specific receptors for dopamine, adrenaline and serotonin, which are located on neuron surface in the spinal cord. This cocktail of drugs replaces the neurotransmitters in healthy subjects, release pathways in the brainstem and activates stimulation of neurons preparing them for coordination of the movement of the lower parts of the body when the time is right.

Five to ten minutes after the injection, the researchers electrically stimulated the spinal cord with electrodes implanted in the outermost layer of the channel, which is called the epidural space. "This way localized continuous epidural stimulation sends electrical signals through the nerve fibers to chemically excited neurons that control the movement of the legs. All that remains is to move the leg, "explains Rubia van den Brand, co-author of the research.

The article in 2009. in the journal Nature Neuroscience, Courtine reported that stimulated spinal cord of rats - physically isolated from the brain - has developed very surprisingly. It started by assignments of sliding leg movements, allowing the earlier paralyzed rats to walk, though not by their own will. These experiments have revealed that the scroll wheel has created sensory feedback that initiated walking. "The back brain" then took over further walking and basic walking happened with no information received from rat’s actual brain. It was a surprise for researchers which led them to believe that animals need a very weak signal from the brain to run the limbs of their own accord.

To test this theory, Courtine replaced the scroll wheel with a robotic device that adhered to the body, which was turned on only when the rat lost his balance, giving the impression that he is healthy and able-bodied spine. This has encouraged the rats themselves to go for chocolate as a reward waiting at the other end of the platform. "They concluded that exercising will encourage movement and speed-up the regeneration of nerve fibers four times. That statement shows that nerve cells, opposite to the previous statements has a great neuroplasticity and regeneration potential.

Courtine called the growth of fibers "new ontogenesis," which is a kind of duplication of child growth phase. Researchers have found that newly formed fibers bypassed the original lesion in the spinal cord and thus allowed the signals from the brain to reach electrochemically awakened spine. The signal was strong enough to start moving on the field, and without the car. This means that the rats began to walk alone, by their own will toward the prize at the end of tracks, fully supporting its own weight on its hind legs.

"This is the world cup of neurorehabilitation," said Courtine. "Our rats have become athletes even though, only a few weeks ago, they were completely paralyzed. I'm talking about complete recovery of voluntary movements. "

In principle, a radical reaction of the rat spinal cord in this type of treatment gives us the reason to believe that the persons with spinal cord injury will soon have some new options for treatment. Courtine is optimistic that the new phase of experimentation on humans will begin within a year or two at The Spinal Cord Injury Center at the Balgrist University Hospital in Zurich.

Discoveries and Technologies Inspired by Animal Abilities

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Although it seems quite impossible at a first glance, people in the future will walk just like insects and jellyfish will read the barcode. The swimmers will wear the costumes modeled on shark skin, and planes will mimic birds. It is going to be the era of useful technology tools developed on the models taken from living nature.

Regardless of the position, walking on the ceiling, a tree or a leaf, insects easily defy gravity and they don’t care whether they move through the rough, smooth, wet or dry surfaces. Insects have inspired researchers at the University of Kiel to invent the duct tape that can withstand the weight of a grown man. Since there is no glue, even after thousand removals it does not lose its adhesive properties.

Using genetic engineering, scientists have created artificial equivalents of millions of tiny hairs that insects have on their tiny legs. Because there are so many of them, insects easily grip and have an "intimate" contact with any surface, and the invented duct tapes are effective and even under the water, and leave no sticky trail. Tapes will not be expensive, and it will be commonly used in everyday life, robotics, and for building spaceships.

The synergy of technology and nature, which no longer play in opposite teams, is the new trend among scholars, and a new discipline, biomimicry, or bioinspiration uses nanotechnology to mimic efficient and sustainable solutions from nature. Therefore, the engineers at the University of York in England, mimicking the horns of a deer in the fight, have made the material that is both extremely rigid and very sturdy, and will in the future be used to make durable materials. Indian snail shell is also extremely durable, and there are already uniforms for soldiers and armored vehicles made using that principle.

Medusa Gives Laser Light

Protein that mimics the light was first discovered in 1961 in the luminous jellyfish, and scientists can now get laser light from living cells, which is highly effective for all forms of light therapy in medicine. Imitation of laser light could improve microscopes, and scanners at the box office or DVD players.

Bionic Car Mimics Goldfish

Mercedes engineers have made a concept car that mimics the form of tropical fish. Due to its advanced aerodynamics the car consumes 20 percent less fuel, and spends three liters to go 100 km.

Nice and Clean in a Dirty Pond

The lotus flower manages to live in dirty water and to nothing lose from its purity. It floats on the water regardless of the heavy rain that cannot soak it. Its leaf is covered with tiny protrusions that make the water to gather in droplets and on that way. The lotus effect has the potential to be applied to all types of cleaning, from shoes to cars.

Windmills Are Working 20 Percent Better Because of Whales

Another interesting application of biomimicry, or imitation of nature was successfully completed when the scientists investigated the behavior of humpback whales.

These huge animals are known for their songs which they spread through the depths of the ocean, but also smooth and agile behavior which allows them to easily move their heavier than 35 tons body. The secret is in the shape of their humpbacks and fin folds that up to 30 percent better utilize power stroke. This knowledge engineers have used to make highly efficient hydraulic turbines, windmills, irrigation pumps, huge fans and propellers. Drawing on the experience of humpback whales, newly designed turbines produce twenty percent more renewable energy.

Cold Air Under The African Sun

Millions of termites fried in the hotness under the African sun, in termite mounds have made cooling system with corridors and chimneys. For the architects in Zimbabwe, it was the inspiration to make a building designed to always maintain the ideal temperature naturally.

Swimsuit Modeled on Shark

The Olympic pool each thousandth of a second counts, and sporty swimsuit inspired by a shark makes a decisive advantage to competitors.

Airplane With Wings Modeled on Birds Is Flying Easier

Mimicking birds and fish, scientists from Penn State University in the United States invented the special airplane with wings that can change shape and position depending on the speed and duration of flight. Similarly like the wings of birds, artificial wings save energy and fuel significantly, and enable faster flight.

The Use and Benefits of Biodiesel

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Biodiesel is a liquid fuel produced from vegetable oil or used oil and grease. Most transportation fuels are liquids, because vehicles usually require high energy density, as occurs in liquids and solids. Biodiesel burns very purely and resembles a standard diesel. It is derived from the oil that is produced from sunflower, soybean or rapeseed oil using methanol and a catalyst. Chemically, it is the mixture of methyl esters of fatty acids. This fuel is a renewable resource because It regenerates at the same speed as being spent. This fuel has a bit lower energy content, but it contains a higher percentage of oxygen which helps it to combust better.

Conventional diesel engines use fuel with 20 percent biodiesel without difficulty, and many of the new engines can also use pure biodiesel. Biofuels do not require the production of a new car or a new car engine, they already have a huge advantage over hydrogen technology which is still at the beginning of the development. In 2010, worldwide biofuel production reached 105 billion liters.

This compatibility with existing engines has prompted many countries to turn to biofuels, confident that they will thus be able to reduce the cost of fossil fuels. The European Union has set itself the goal to use 6 percent of biodiesel by 2010, which will mean a five-fold increase in growing plants that produce ethanol.

The Advantages of Biodiesel Compared to Diesel

Technical aspects:
• Provides better ignition and lubricity which means greater engine efficiency and duration
• Safer handling and storage: flash point of about 150 ° C - fossil diesel about 70 ° C
• Requires no modifications to the engines
• It is not necessary to change transport and storage systems in order to use biodiesel
Ecological aspects:
• Reduced emissions of greenhouse gases, particulates and aromatics: CO2, CO, SO2, NO2, soot, benzene, toluene
• Nontoxic
• Biodegradable
Energetic aspects:
• Renewable basic raw materials and the use of already used cooking oils and fats
• Reduces the need to import oil and the risk of supply
Economic aspects:
At the macroeconomic level, the development of biodiesel production affects the following indicators:
• Employment
• The increase in industrial production
• Additionally increasing funds to agriculture
• Contribution to the economic development of rural areas
• The increase in foreign exchange reserves
• Reducing energy parameters depending on external factors.

Why Biodiesel?

• The need for increased security in the supply of liquid fuel for the transport sector and agriculture using renewable sources;
• The need to be used in diesel engines that don’t pollute the environment and at the same time does not require modifications to the engine and can be blended with fossil diesel;
• Provides users with reliable fuel at a lower price.
• Reduced emissions (especially CO2) that participate in greenhouse gas emissions and their impact on global climate;
• By ratifying the Kyoto Protocol, the EU committed itself to reduce total emissions by 8% by 2012 compared with the level of the year 1990
• EU member states must ensure that the minimum proportion of biodiesel and other renewable fuels is in its markets;
Reference value of the goals set according to Directive 2003/30/EC (calculated based on the total energy content of gasoline and diesel fuel): 2% by 31st December 2005, 5.75% by 31st December 2010, and the European Commission determines further progress.

Selling Equipment for Biodiesel

The innovation is based on a flexible and modular mobile transesterification technology, which allows the conversion of restaurant waste oils and fats to get quality refined oils and fats according to standard EN 14214. Built-in modular units are also necessary for evaporation and distillation of methanol, which enables effective and maximum use of unreacted methanol.

Second-generation biofuels are produced from sustainable feedstock. Sustainability of a feedstock is defined, among others, by availability of the feedstock, and impact on biodiversity and land use. Many second-generation biofuels are under development such as Cellulosic ethanol, Algae fuel, biohydrogen, biomethanol, DMF, BioDME, Fischer-Tropsch diesel, biohydrogen diesel, mixed alcohols and wood diesel.

The problem of energy deficiency is raising in all industrial and economical fields, ant threatens to impact industrial, science and technology progress. Biodiesel is considered a very advanced technology achievement which has the potential to change human life in the sense of overcoming the world energy problem.

The Acne causing bacteria - Propionibacterium

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Acne is the most common skin disease seen as small bumps on the skin. Acnes are always seen as tiny disasters on the skin and the people prone to acnes develop a complex inferior to those with a clear skin. Especially young people under teen groups just dislike seeing that tiny ball on their face. One with the acne trying to find out ways in getting rid of it seeks many advices from peers and also consults dermatologist. The general suggested ways of combating with acne includes change in diet, avoiding oily food stuffs, consuming plenty of water and fluids, washing the face frequently, use of soaps and face wash solutions prescribed by the doctor, selecting the correct cosmetics suitable for the skin, taking antibiotics prescribed by a dermatologist, washing the hands before touching the face, avoiding hair and the facial skin contact, using clean tissues and towels to wipe the face, avoid squeezing the pimple and the list goes on. Also, now-a-days there are many skin clinics emerging with latest skin care technologies promising a clear skin devoid of pimples and removal of scars developed as a result of pimples.

The different important factors contributing to the development of acne are the nature of the skin, sex hormones and the bacteria. In general, nature of the skin is classified as normal, oily and dry skin and people with oily skin are more prone to acnes. Both men women develop acnes as a result of change in their sex hormonal levels.

The next major causative agent responsible for acne is the bacteria. Yes, human skin acts as a habitat for many bacteria and virus. Though most of them are harmless some of them cause skin infections. Among these bacteria is the Propionibacterium (Gram positive, rod shaped), the microbe behind those acnes on the skin. The occurrence of pimples is also considered as the immune response developed by the individual against the action of this bacterium. The Propionibacterium depend upon the sebum secreted by the oil glands beneath the skin and the cellular debris present in the follicles and the pores of the skin for their nutrition and growth. This bacterium is also known to secrete enzymes that are of digestive nature which causes disruption of cells present in the follicles of the skin. The blocked pores of the skin, the accumulation of the dead cells and the excess secretion of the sebum by the oil glands acts as the perfect environment for the bacteria to grow and multiply and influences the formation of those small bumpy structures on the skin.

The medication prescribed in treating acnes is limited whatever may be the cause for the development of them. It includes prescription of topical creams and gels with antimicrobial property to be applied on the affected area of the skin and antibiotics to be taken orally. There is also some disadvantage in using topical gel. The applied gels tend to get absorbed by the skin and enter the blood stream causing problems like diarrhea, colitis receiving clinical attention.

With the availability of though effective but limited treatment methods to the largely affected population, the work by the research team from the university of California and University of Pittsburgh states the discovery of a virus obtained from the human skin is effective against the acne causing Propionibacterium and reduces the acne effect by killing this bacteria. Human skin acts as the habitat to both the Propionibacterium and the discovered virus, the bacteriophage. The virus is found to cause no harm to the humans but is designed to kill the bacteria and hence it is considered as an ideal bacteriophage for the acne causing bacterium.
The genomic sequence of the virus, its size, its potential to kill the bacteria seems to be the attracting factors for the scientists to use them as an effective vehicle to kill the Propionibacterium thus reducing the development of acnes. Also the scientists state the designed virus’s specificity to the acne causing bacteria makes it a valid tool against the Propionibacterium. As a further progress to this finding the scientists are trying to extract and study the active protein of the virus responsible for the destruction of the bacteria which will pave way for the development of active product instead of using the virus as such in treating acnes.

The ongoing researches in the development of safe, effective and promising treatment methods for acne will always be an ideal gift to the acne sufferers.

Protein Against Mice Aging : BubR1

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BubR1 Protein:

Incredible 10-year journey, which had started by the discovery of laboratory raised mouse that get old fast, led scientists to trace the protein that potentially protects animals from cancer and other effects of aging. Although there are still many uncertainties related to that protein called BubR1, there are evidences that the so called protective chromosomes can improve health.

Aneuploidy Causes Cancer?

Jan van Deursen, a biologist from Mayo Clinic in Rochester (Minnesota), who conducts research on tumors, and his colleagues were originally interested in studying the common features of different tumors that are called aneuploidy tumors. Aneuploid cells have an excess or deficiency of chromosomes. Most tumor cells fall under this category, but it remains unclear whether aneuploidy causes cancer indeed.

The Experiment

Van Deursen, along with former student Darren Baker, using genetic modification of mice decreased the production of BubR1 protein that helps cells with chromosome separation after division. After reduction of BubR1 chromosomes can not be properly separated into identical daughter cells, which is why some of the new stations do not have the correct number of chromosomes. Van Deursen, Baker and colleagues have questioned whether tumors will develop in these mice.
To the great surprise, instead of mice full of tumor cells, they have got animals in which the aging process was significantly accelerated. "These mice are obviously strongly different from normal mice," said Baker, who is currently engaged in the biology of aging at the Mayo Clinic. Last year, they found that these mice may help you live longer and healthier by removing old cells, and those cells whose genetic markers indicate aging.

BubR1 gene mutations are extremely rare in humans. This syndrome is characterized by a wide range of aneuploidy, premature aging and increased risk of breast cancer. It seems that the low amount of BubR1 protein is very harmful.

On the other hand, excessive amount of the same protein has a positive effect on health. In the study published in the journal Nature Cell Biology biologists argue that mice, in which the level of BubR1 is highly increased using genetic engineering, are less prone to the development of tumor cells. For example, when normal mice were exhibited to a chemical that causes tumors in the lung and skin, all of them got a cancer. However, only 33% of mice in which extremely high levels of BubR1 are found, developed a cancer under the same conditions. They have found that in these mice the fatal forms of cancer occurred about two years later than normal, and only 15% of the genetically modified mice had died of cancer, compared with 40% of normal.

Mice Run Like Olympians

At the same time, the animals with markedly increased levels of BubR1 lived on average 15% longer than the control group, and they looked like real Olympians on a treadmill, running approximately twice as much as normal mice (200 meters compared to 100 meters). Baker, Van Deursen and colleagues therefore hypothesized that BubR1 does not affect the prolongation of life only by reducing the risk of cancer, and that this hypothesis needs to be checked.

It is of great importance to know why the disorganization of chromosomes can accelerate aging, says Dai Wei, a cell biologist at the University Langone Medical Center in New York. Although aneuploidy seems harmful, studies does not give that kind of results of its effect on animals. "We found that a low level of aneuploidy, such as the one in Van Seurden’s healthy mice, leads to a large number of tumor cells, and not to lower," says Cristina Montagna, molecular geneticist at the Albert Einstein School of Medicine in the Bronx. She and her colleague Jan Vijg collaborate with Van Deursen in study of brain BubR1 in mice. A possible explanation of their findings is that very low as well as very high aneuploidy protect of tumors because the highly aneuploid cells are so damaged that they don’t have the ability of quick division.

Still, there is hope that Van Deursen and colleagues will find a new drug that will slow aging. "We certainly have not found adverse effects of excess amount of BubR1 protein," said Paul Hasty, who studies aging and recovery of DNA at the Center for Health Sciences at the University of Texas. He added that it is necessary to reveal the mechanisms by which BubR1 achieves the desired effect, but this could be a first step in discovering the treatments that could delay aging and possibly prevent the development of cancer.

The Injections That Do Not Require a Needle

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Pain during vaccination and fear of needles could completely disappear in the near future, writes MIT news.

Scientists at MIT have constructed a device by which the content of the vaccine is injected under the skin of the patient without the use of hypodermic needles. Simply put, it is a device that injects contents of vaccines by “jet" drive, without causing any pain. The advantage of this invention is that it can inject different amounts of vaccine at different depths.

Less Accidental Injuries

Scientists say the invention, along with many other benefits, can help prevent accidental injuries that occur daily handling with needles. U.S. Centers for Disease Control and Prevention estimates that hospital staff accidentally injures with needles of various vaccines even 385 000 times a year. Of course, in most cases it is only a tiny prick, but with this large number of accidental puncture there is a significant risk of transmission of certain serious diseases. From a multitude of other benefits should be noted that giving the vaccine without needles can significantly ease the life of people that have to be injected daily with medications such as insulin.

"If you have a fear of needles or must daily inject a drug, there is a good chance that you will sometimes avoid the prescribed treatment because of fear or discomfort," says Catherine Hogan, a scientist at MIT's Department of Mechanical Engineering and a member of the team that constructed this device. "We believe that this invention has an extraordinary potential in helping patients in overcoming phobias associated with medical needles," says Hogan.

Attempts To Replace The Needle

In the past few decades, scientists have tried various alternatives to hypodermic needles. Nicotine patches, for example, release slowly the drug, which then enters the body through the skin. But the problem with this solution is that from the patch only molecules that are sufficiently small may be released, as they have to be able to pass through tiny holes in the skin (pores). By replacing the hypodermic needle with patch, the range of drugs that patients can receive is therefore significantly limited.

More recently, in medicine are increasingly being used "big" drugs based on proteins that scientists had to find more efficient ways to bring in patients organisms. One of these methods is the injection of jet injection, that is able to inject painlessly various types of drugs at high speeds under the skin. Although in today's market there are several similar devices, Hogan points out that each of them has some significant drawbacks.

The Entry Under The Skin

MIT team led by Professor Ian Hunter, unlike all the previous cases, constructed the jet injection which was able to inject different doses of drugs at different depths and all this in a very reliable manner. The basis of the device is a mechanism called Lorentz-force actuator - a small but extremely strong magnet surrounded by a coil of wire that is connected to the piston within the ampule filled with medicine. When the current is released through the device, it activates a magnetic field which then generates a force that ejects the drug through the nozzle out under very high pressure and at very high speed (the speed of drug ejection is close to the speed of sound). The nozzle through which the drug is "fired out" is as wide as the proboscis by which mosquito stings their victims.

The speed of injection of the drug can be controlled by the amount of current that is passed through the device. The injection process is usually carried out in two phases. In the first stage (high pressure phase), the device ejects the drug under extremely high pressure. This is necessary in order to inject the drug under the skin and in order to reach the desired depth. In the second stage (low pressure phase) in which the pressure is significantly lower, the drug will be expelled in a slow stream to be absorbed by surrounding tissues properly.
When testing the device, the researchers noticed that the quantity and depth of drug injection significantly depends on the skin type on which the device is used.

"If, for example, using this device we wish to vaccinate a child, it would be much less pressure than when they tried to inject the drug under adult skin," says Hogan, and continues, "we can easily control the pressure at which the drug is injected, and that's one of the biggest advantages of this device. "

Telomerase Enzyme Extends the Lifespan of Mice

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Scientists are trying to inspire give the humanity the gift of immortality. In the latest attempt to eradicate death, the researchers injected the healthy mice with dose of telomerase, an enzyme that our chromosomes (and therefore our cells and bodies) maintains "young". With this therapy, scientists were able to extend the life of mice by 24%, and best of all is that the therapy produced absolutely no unwanted side effects. At least not yet.

Shortening of Telomeres is Responsible for Aging

As man gets older, the cells are getting older too. Dying cells are replaced by new ones by cell division, but this process is not without side effects. After each cell division, DNA molecules are shortened at the end of chromosomes (regions called telomeres). At one point, when the telomeres are shortened to a certain extent, this piece of DNA signals the cell that it is time to stop further division. Cessation of cell division prevents the formation of new tissue, and therefore, this process leads to the destruction of tissues build of those cells. As a result, these tissues are rapidly deteriorated and, to cut a long story, this process seems to be the reason people do not live forever. But maybe not for long.

The Molecular Cocktail

Scientists claim that they have found a molecular cocktail that, at least in mice, act like an elixir of youth. The scientists injected the telomerase gene in mice, which has slowed down the aging process by lengthening the telomeres and thus allowed cells to divide much longer time than it would be in a normal state. Researchers injected the molecular cocktail to the one-year mouse (in the mouse world, this is considered an adult) and two-year mouse (briefly - an old man). Lifetime of one-year mouse, by this therapy is prolonged by 24%, while the percentage of two-year mouse was slightly lower - 13%. But besides they lived longer, the mice benefited from this therapy in many other ways. It seems that the therapy had a very positive effect on a variety of conditions that are commonly associated with aging, including insulin sensitivity, osteoporosis and physical coordination.

It should also be noted that the second group of mice was injected with inactive form of telomerase, which had no effect on lifetime extension. This confirms that it is the enzymatic activity that has resulted in the extension of telomeres crucial to find an elixir of youth. The study (published in the journal EMBO Molecular Medicine) was led by the Spanish scientist Maria Blasco from the Spanish National Institute for Cancer Research.

The Use of Viruses as Gene Vectors

This therapy included the replacement of the particular gene in the viruses with the gene for telomerase. This had several advantages. First, viruses are great way to enter the body and they infect a large number of cells. Insertion of telomerase in a small number of cells would probably not have any significant effect on prolonging the life of "infected" body. Second, in this way, the gene remains active for years and, the last, the DNA from the virus did not interfere with DNA from mouse cells. In previous attempts with this type of therapy, there was a considerable risk that the virus infected cells will turn into tumors. For example, in one of the studies, this therapy has caused leukemia in two of nine mice.

The very idea of life extension using telomerase is not new. Scientists have already added telomerase to cultured human cells allowing thus at least 20 new divisions and significantly extent of their lifespans. Mice, which were artificially enabled to produce telomerase, lived 40% longer, and in these mice were observed greater levels of glucose tolerance, better coordination, and less tendency to other infections compared with normal mice. Still, the same genetic modification in humans is not yet a realistic option.

The Answer Is Maybe Near

Aging is a very complicated process that is not yet fully understood. It includes so many factors that it is quite likely that today we are not even aware of the existence of certain important characteristics of the aging process. But if the key to our aging (and of course, death) is just telomere shortening, then the answer is very near, according to recent, above mentioned findings. In short, the point is that we always have enough time to find new drugs for, as de Grey said, "curing the disease called death." If we reach the destination of this exciting journey, it might have been the last step toward immortality.

Analysis of Bacterial Biomass Growth and Metabolite Accumulation

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Determining Stoichiometric Coefficients
of Metabolites in Glucose Fermentation
Sergey P. Klykov and Vladimir V. Kurakov
03/01/2013
http://www.bioprocessintl.com/multimedia...87894a.pdf

Our proposed structured deterministic model describes the kinetics of biomass growth. Metabolism is not worse than other models, but rather provides a simpler mathematical approach. Our model allows for analyzing the chemical stoichiometry of biological processes. Such analysis provides for the economic calculation of the industrial processes of cell culture and a real forecast of the parameters.

Also see:
http://www.biotechnologyforums.com/thread-1809.html

Chinese Scientists Made Brain Cells From Urine

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Chinese scientists have developed a new technique by which the cells from urine are reprogrammed to become immature brain cells that can form several types of functional neurons and glial cells. This technique, published in the journal Nature Method, could become particularly useful for the study of cellular mechanisms in neurodegenerative disorders such as Alzheimer's or Parkinson's disease. Also, using "reprogrammed" cells from urine, the new drugs could be tested for two of the above, and many other diseases.

Viral Vectors Cause Harmful Mutations

Stem cells could significantly help in the treatment of various serious diseases, but their collection from human embryos is almost inextricable ethical dilemma. Fortunately, science is now so advanced that it is possible to take common body cells from adults and to make stem-like cells out of them. After that, from those new stem cells, all the other types of body cells could be derived. During this process, genetically modified viruses are commonly used to deliver genes that control the cell nucleus and are inserted into the chromosomes. After that, the genes that make cells pluripotent or reprogrammed are activated, making the stem cell from which it is now possible to make any type cell.

Let’s simplify this process by an example. Four years ago, U.S. scientists took skin cells of 82-year old patient who suffered from severe sclerosis. With the help of genetically modified viruses the cells were then reprogrammed into neurons responsible for motor processes. So, the cells that scientists "created" from simulated stem cells could help them in the understanding and treatment of many diseases. Being said that the patient receives his own cells, the risk of a reaction of the immune system is eliminated, and therefore, doctors are hoping that this approach will lead to success in future cell transplantation therapies. However, although at first glance it does not seem like it, the whole procedure contains some significant drawbacks. For example, it appears that the process of reprogramming cells destabilize the genome and causes various mutations. If you are trying to cure the patient with stem cells that will cause harmful mutations in its genome, then the whole process is ultimately useless.

The New Method

Last year, Dr. Pei Duanying from the Chinese Academy of Sciences with the group of co-workers published a research paper in which they presented a pretty interesting research on human urine. Specifically, Pei and his colleagues concluded that urine contains cells like skin cells (which came in the urine from the renal tubules) that can be successfully reprogrammed into neurons, glial cells, liver cells and heart muscle cells. After a year of intensive work, Chinese scientists have mastered the process and made it faster, more efficient and more reliable.

In the latest study, the cells were isolated from urine samples of three donors (donors were 10, 25 and 37 years old) and were converted into stem cells, from which neurons were developed. After that, the reprogrammed cells were grown in petri dishes, and developed in mature neurons that can generate nerve impulses. From the stem cells, they have also made oligodendrocytes and astrocytes, the two types of glial cells, which are found in the human brain. Once they raised the desired cells, neurons and astrocytes were transplanted into the brains of newborn rats, and a month later they noticed that their grown cells are still alive in the bodies of animals. While this is in itself quite a success, the researchers emphasize that the actual critical period is yet to come - it is necessary to see how long this cells can survive and, in particular, could they be incorporated into the neural network and become fully functional.

This is not the first time that one cell type was converted to another, and that the cells had not previously been brought into pluripotent stage. Two years ago, scientists at Stanford University directly transformed connective tissue cells into neurons in mice. However, this technique still has several important advantages compared to those used by the Stanford team.

Instead of using a virus as a vector for reprogrammed genes, Chinese researchers used a tiny piece of bacterial DNA that can replicate itself. This approach not only speeds up the whole process, but also eliminates the need to reprogram the genes integrated into the chromosome. This process has often caused genetic mutations, and that way made the whole idea of reprogramming cells unusable. However, it is still not clear whether the reprogrammed cells using bacterial DNA are less prone to mutations and what the real benefits of this approach are.

Of the remaining benefits of this new approach we should emphasize the fact that it makes the whole procedure of the creation of new cells much easier and much less invasive. This simply stems from the fact that cell samples now can be derived from urine and not from blood or biopsy. The next step for researchers is to create neuronal cells from urine collected from patients with Alzheimer's, Parkinson's and other neurodegenerative diseases. Since the whole approach was developed precisely in order to treat these conditions, it is crucial to find out whether and how this non-viral way of reprogramming cells affects the incidence of harmful mutations in the genome of the patient.

Hydra Polyp Holds The Secret of Long Life?

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Why do people get older? Why do they die? Is there life without aging process? These and similar questions have fascinated scientists for centuries, and now researchers from Germany's Kiel accidentally came very close to the answers to these questions, reports Science Daily portal. Specifically, studying the longevity of polyp hydra, scientists are completely unexpectedly found a link with aging in humans.
Research conducted by joint efforts of scientists from the University of Kiel and the University Medical Center Schleswig-Holstein (UKSH) was published in the journal Proceedings of The National Academy of Science (PNAS).

Hydra Polyp Is Immortal

This tiny polyp lives in deep water and, according to current knowledge, it shows no signs of aging, which makes him literally immortal. Despite the expectations, behind the immortal life of this organism is actually pretty simple biological explanation - hydra are asexual organisms that reproduce by the process of "budding". It is a relatively simple process in which an individual develops so called buds that remain attached to the mother until the moment when they become quite mature. Being asexual, buds are actually clones of mother, so, once separated they transfer always the same genetic material. The basic assumption of this form of reproduction is that each polyp contains stem cells capable of virtually unlimited proliferation. Without these cells, these organisms would no longer be able to breed. Because of its longevity, Hydra was the subject of many studies during recent years, but this is the first time that an association was found between polyps immortality and aging in humans.

When people get older, an increasing number of stem cells lose their ability to replicate, and thus the ability to produce new cells. When a specific tissue is old enough, it can not be regenerated, which is the main reason, for example, for muscle atrophy in the elderly. Also, older people often feel weakness because the aging process affects their heart muscles. If in some way, we had an impact on slowing down these side effects of aging, people would be able to feel better physically much longer than is now common. The study of tissues in organisms that are full of active stem cells during entire life, may provide us with a better insight into the aging of stem cells in general. Of course, the ultimate goal of this type of research is to understand the aging process in order to change it in some way.

FOXO Genes

"To our great surprise, the search for genes that enable hydra to be immortal has brought us to the so-called FOXO genes," says Anna-Maree Bohm, a student in the doctoral study and co-author of the article in which the results of research are presented. FOXO gene exists in all animals and humans, and scientists noticed it long ago. However, until now there was nothing known about why older people have less stem cells that are, in addition, less active, which biochemical mechanisms are associated with this process, and what role does FOXO gene have in aging process. In order to find this gene which is clearly important, the researchers extracted stem cells from hydra and mapped all of their genes.

The research team from University in Kiel studied the FOXO gene in several different genetically modified polyps - hydra with normal FOXO gene, with inactive FOXO gene, and with enhanced FOXO gene. Based on several experiments, the scientists were able to prove that organisms without FOXO genes have much less stem cells than the other organisms. Interestingly, drastic changes in the immune system were observed in these organisms. "Drastic changes are similar to those of hydra, and are often observed in the elderly," explains Philip Rosenstiel from the Institute for Clinical Molecular Biology, UKSH, who, along with his team of experts, participated in a particular study.

"Our research team has shown that there is a direct link between FOXO genes and the aging process. FOXO gene is especially active in people who have suffered over one hundred years old and it was our only additional evidence that this gene plays an important role in the aging process, not only hydrated, but also in people, "says Thomas with Bosch the Institute for Zoology at the University of Kiel, senior author of the study.

However, currently there is no way to check this hypothesis indeed, since that would require such an undertaking, as genetic manipulations with people, the limit that science has still not exceed. Nevertheless, Bosch points out that the results of this research are a big step forward in understanding how and why people get older. Since further studies in humans are not possible, scientists will in the near future be devoted to the study of ways in which FOXO gene acts by studying the hydra polyps.

Induced Pluripotent Stem Cells (iPS) - Blood Cells into Embryonic Pluripotent State

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iPS or iPSC are derived from skin or blood cells that have been reprogrammed back into an embryonic-like pluripotent state that enables the development of an unlimited source of any type of human cell needed for therapeutic purposes.

The discovery of embryonic stem cell (ESCs) from mouse as well as from human embryos has created more interest due to the pluripotency of the cells, that is their ability to produce any kind of cell in the body. They were engaged to transform the area of regenerative medicine in which unhealthy tissues or organs in an individual could be replaced by transplantation of cultured cells or an in-vitro produced tissue or organ. On the other hand its utilization has often been restricted by the fact that, if utilized for transplantation, the source of these cells (blastocyst from the donor) that is from a genetically dissimilar but of a similar species (allogenic), will be most probably recognised as non-self by the immune system of the recipient. Therefore utilizing the cells derived from embryonic stem cells for transplantation from an allogenic donor, may require a lifelong immunosupression and the problems associated with it. Further the reality that embryonic stem cells normally necessitate the utilization and destruction of blastocyst staged human embryos has important ethical issues.

The above hindrances have been resolved by an important breakthrough made by the team of Shinya Yamanaka in Japan. For about a period of ten years, Yamanaka worked on the likelihood of transforming somatic cells of adults into pluripotent stem cells. To carry out that task, he concentrated on a set of genes that were exclusively or greatly expressed in embryonic stem cells. The researchers in his group initially utilized retroviruses to over-express twenty four genes in fibroblasts of mouse and astonishingly after a few weeks afterwards they found that colonies greatly alike to usual mouse embryonic stem cells appeared in the cluster plates. In contrast to the initial fibroblast population, the resultant cells could be indefinitely maintained in culture and was able to function as embryonic stem cells. Furthermore they went still far to reveal that only a combination of four out of the twenty four genes, named oct 4, klf 4, sox 2 and cMyc (all of which code for the proteins that function as transcription factors to control the expression of diverse sets of genes) were adequate to induce nuclear reprogramming of somatic cells and to revert them to a primeval, pluripotent state. To differentiate these novel cells from their embryonic counterparts, they were mentioned as “induced pluripotent stem cells” or “iPS cells”. Later research showed that similar ideology could be applied to reprogram human somatic cells, a key move towards the long wanted utilization of pluripotent stem cells in the field of regenerative medicine.

Even though the initial investigations utilized the fibroblasts, scientists have revealed that various kinds of cells including cells from liver, stomach, pancreas, and also blood lymphocytes can be reprogrammed to become induced pluripotent stem cells. Further studies by the scientists have revealed that almost any kind of cell could be utilized as a potent target for reprogramming, excluding the thought that a rare progenitor or stem cell was the foundation for the production of an iPS cell. In an effort to recognize the biology of the reprogramming mechanisms, various researchers have tried to decode the function of each individual reprogramming factor. Even though yet not wholly understood, it revealed that cMyc gene has a distinct role from the other 3 genes, oct 4, klf 4, and sox 2. During the initial stages of nuclear reprogramming, cMyc is accountable for inducing the down regulation of genes usually expressed by differentiated somatic cells and at the same time initiating cellular metabolic changes. Oct 4, klf 4 and sox 2 seems to be significant in maintaining and establishing the stem cell gene expression program. About the dynamics of the reprogramming mechanisms, it is a comparatively slow process, utilizing a minimum of about eight to ten days of sustained expression of the reprogramming factors. In that period the endogeneous loci of genes that are significant in establishing a stem cell programme are remodelled to become actively expressed, whereas the expression of the reprogramming genes introduced through the viral vectors become silent. This feature might be principally essential for appropriated differentiation of the induced pluripotent stem cells.

It is well known that the enduring presence of the reprogramming factor transgenes (foreign genes) inside the genome of the established induced pluripotent stem cells, in which the reprogramming genes are not regulated by their usual or natural regulatory elements but by a viral or other regulatory elements, presents a strict complication for the use of this technology in the clinical field. But there is a huge potential for induced pluripotent stem cell research, where adult somatic cells such as skin, fibroblasts and peripheral blood cells can be collected effortlessly from any individual and can be utilized as the source for the production of personalised pluripotent stem cells. Subsequently, induced pluripotent stem cells can be engaged for the production of definite tissues or organs that can serve for the learning of any disease, monitor for novel therapeutic drugs or utilization in cell replacement therapy.

Exemplary illnesses that benefit from this technology includes, Amyotrophic lateral sclerosis (ALS), Inflammatory bowel disease (IBD), alpha -1 antitrypsin deficiency (AAT), Alzheimer’s Disease, Cystic fibrosis, Diabetes, Parkinson’s disease, cancer and so on. The practical revelation that induced pluripotent cells were in fact pluripotent was their capability to contribute to all kinds of tissues when injected in to immuno-compromised mice to form teratogens (tumors that include tissues from all 3 primary germ layers). Notably the mouse induced pluripotent cells were able to produce a whole mouse when injected into a mouse blastocyst. Pluripotent stem cells, similar to embryonic stem cells, have specific epigenetic prospect, which are significant for preservation of pluripotency. Production of induced pluripotent cells that are induced by Transcription factors requires total alteration of the epigenetic form of somatic cell into an embryonic stem cell like state. Therefore gathering evidence specifies that epigenetic processes not only play vital roles in the induced pluripotent cell production process, but also have an effect on the properties of reprogrammed induced pluripotent cells. Considering the functions of several epigenetic factors in induced pluripotent cell production also adds to our information of the reprogramming processes.

Induced pluripotent stem (iPS) cells have the prospective to alter drug discovery by supplying physiologically suitable cells for poisonous compound identification, target validation, compound screening, and tool discovery. The technique for producing induced pluripotent cells is marching speedily, as is the range of cell types that can be differentiated. Tissue-specific cells derived from induced pluripotent cells are presently being investigated by the pharmaceutical diligence for their use in recognition of cardiotoxic and hepatotoxic substances as therapeutically applicable systems for modelling cardiovascular, neurodegenerative and metabolic disorders, as well as for production of individual specific cell kinds.

Scientists Created Mosquitoes That Cannot Transmit Malaria

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One of the most common and most effective ways to fight the spread of malaria is to control mosquitoes that carry them from one man to another. Unfortunately, this way of fighting turned out to be ineffective as the epidemic of malaria can not be fully controlled. Spraying mosquitoes in the past few years has been less effective because the mosquitoes quickly developed immunity to pesticides. A similar process occurs with the very parasite that causes malaria, which repeatedly developed resistance to various drugs that came from science labs.

But it is possible that science finally has a solution that does not mean the destruction of large populations of mosquitoes, the carriers of malaria, according to Ars Technica. In fact, several years ago began testing in the wilderness, during which the mosquitoes that transmitted dengue fever were infected with a special type of bacteria that prevents the spread of parasite. In a recently published study, a team of scientists published almost sensational results of long-term experiments – they were able to create genetically modified mosquitoes, which, even if they pick up the parasites of malaria, are not able to transmit it to humans. Very simply put: scientists "forced" the Mosquitoes to eject specific antibodies that destroy the parasites that cause human malaria, every time they feed on human blood.

Malaria Parasite Camouflage

Various antibodies and vaccines in the past have proven to be fairly ineffective methods of treatment and prevention of the spread of epidemic malaria. Vaccines have been indeed ineffective because the Plasmodium falciparum, a parasite that causes malaria, showed remarkable ability to develop resistance to almost every vaccine that scientists have tried to develop. This parasite rapidly develops immunity to vaccines, completely changing the proteins that are found on its surface, and all this in order to "confuse" the antibodies. This method of parasite camouflage is possible only after the Plasmodium falciparum has already reached the body of the host.

Antibodies That Attack The Parasites of Malaria

Mosquitoes, on the other hand, have no immune system based on the antibodies like it is the case in humans, which means that the parasite does not have to use all sorts of tricks while in the body of mosquito. For of this reason, scientists have managed to create the special type of antibody that recognizes the structure of proteins of malaria-causing parasite, and is specific for life stage in which the parasite is, while his host is a mosquito.

Antibodies that attack the parasites of malaria are often complex combination of four proteins (two heavy and two light chains). To avoid a rather laborious process of inserting a variety of genes for the entire combination of four proteins that make antibodies, researchers have developed a compact version of the antibody, which contains only one gene with combined heavy and light chains. After this, scientists inserted two genes that encourage compact development of antibodies in the special places of mosquito genome. To limit the impact of antibodies to the mosquitoes, the researchers ensured that the antibody genes activated only after a mosquito ate his first meal and came in contact with the bloodstream of the victim.

No Risk for Mosquito Population

As for the risk of this genetic modification of mosquitoes will harm them, scientists claimed tests have found that fear misplaced. For female mosquito, genes with antibodies had absolutely no effect, and the males were with minimally shortened lifespan. It is important to note that this shortened life span in no way affects the reduction of mosquito population, since the genetically modified mosquitoes can easily experience a period of sexual maturity. So, the new genes didn’t practically do anything bad to mosquitoes, and those are definitely good news for scientists.

If it is to believe the results of this study, scientists have finally succeeded. To use a metaphor, from the malaria mosquito they managed to create a hotel in which the parasite can check-in, but has no way to check-out. Once Plasmodium falciparum enters the body of mosquito, it must find a way to get the salivary glands in order to be spread to other organisms. Specific genes inserted into the genome of mosquitoes were able to prevent this migration, and in this way they practically stopped from being an important link in the chain of spread of epidemic malaria.

Uses and Benefits of Cochlear Implants

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The cochlear implant is a sophisticated device that is installed in the inner ear, in order to enable the deaf persons to hear again. Cochlear implant replaces the lost function of the inner ear by receiving the sounds, and then using the speech processor converting the electrical signals. These electrical signals are transmitted through the electrodes embedded in the inner ear to the auditory nerve and then further to the brain centers. The result of this advanced technology is giving deaf individuals contact with the sound, and by proper rehabilitation, the understanding of the language and the development of speech is achievable. Children with good rehabilitation success can attend school.

Elements of Cochlear Implant

A cochlear implant consists of an inner and outer part. The inner part consists of electrodes that are surgically implanted in the inner ear. Surgical procedure for installing the cochlear implant does not differ significantly from normal microsurgical operations of the ear. External component of the cochlear implant consists of a microphone and speech processor. Speech processor enables the conversion of sound energy received via microphone into electrical signals, which are then transmitted through the electrode to the nerve fibers in the auditory nerve, and further to the hearing centers of the brain cortex. In this way the patient becomes aware of the sound and is able to habilitate during rehabilitation for independent and unobstructed auditory-verbal communication. Speech processor can be placed behind the ear or worn around the neck.

All elements of the system for cochlear implants are made of natural materials and each product undergoes a rigorous medical and technical tests.

Candidates for Cochlear Implantation

Cochlear implants can help almost all deaf children up to 8 years old, and adults who once had developed speech, and then completely lost their hearing. In children older than 8 years and adults who have not been in contact with the sound, a cochlear implant can provide only a sense of sound, but not understanding of the speech. Consequence of many years of living in complete silence is loss of ability of hearing centers in the brain to create sound pictures, and therefore, the understanding is persistently disabled. This phenomenon occurs because the aging brain loses its plasticity and ability to process the sounds. For this reason it is necessary to install the cochlear implant as early as possible.

Preoperative Diagnosis

In the preoperative evaluation of the patient, the attention is paid to the whole patient, and not just a state of hearing.
Thus, except for audiology diagnostics, the patient must be reviewed by surdologist , neurologists, psychologists, ophthalmologists, and radiologists in order to assess the condition of the inner ear. After the set of analysis, the decision is made about the possibility of installing the cochlear implant.

The Operations

Installation of cochlear implant is a microsurgical operation that takes about 3 hours. During surgery, electrodes are placed in the inner ear, and during the operation, we examine the activity of the electrode set. After receiving results of the measuring that show that the implant is functional, the operation is finished. The postoperative course lasts seven days, when the stitches are removed and the patient discharged from the hospital.

Programming Implants

After the surgery, the implant is not functional. 6 weeks after the surgery the activation of implant is made by linking the implant that is surgically fitted to the speech processor which is located behind the ear or worn on the body. Simultaneously, the hearing map is constructed, and the minimally and maximally bearable sound is determined. That is the range within which the cochlear implant will work. This range extends by auditory training, and in the following programming sessions is getting bigger and bigger. Programming is conducted every month in the first year and every second month in the second year after the operation.

Rehabilitation

After installing the cochlear implant, the rehabilitation is necessary. Cochlear implant itself does not mean the patient can communicate smoothly. In the course of rehabilitation treatment, an acoustic memory is formed and the patient begins to communicate. Many patients after the rehabilitation treatment can attend regular school and, besides that, they can even use the phone.
In short we can say: "A cochlear implant is not a miracle, but it allows the miracle to happen."

Salmon - Now Genetically Modified!

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Salmon is one of the favorite and common fish that we are consuming now. Statistics state that commercial production of salmon exceeded 3 million tons in the year of 2010. According to the UN, as of 2007, the commercial salmon production accounts to about 70% of the total salmon consumed. Commercial aqua culture industry, which is now in the path of a tremendous growth, is facing a problem to meet the ever increasing needs for the fish. It is because salmons could be grown only during spring and summer. Also, due to the unique life cycle of salmons and increasing fishing, the salmon population has been found depleting over the years.

In order to speed up the production of salmon, Aqua Bounty Technologies developed the genetically modified salmon. Aqua Bounty Technologies is a biotechnology firm who does research and development in the commercial aquaculture field. The genetic modification was performed on the Atlantic salmon (Salmo salar). They added growth hormone regulatory gene form Pacific Chinook salmon (Onchorhynchus tshawytscha), which is the largest of the salmons. They also introduced a regulator protein gene from ocean pout (Zoarces americanus). They named the genetically modified salmon as, Aqu Advantage, a trademark. The genetically modified version of the fish can be grown throughout the year and also twice as faster than the common salmon. It weighs more than twice the conventional salmon too. It requires 10% less feeding than the usual ones. The company calls the modified salmon is twice better than the unmodified salmon, without a compromise in size due to the genes from the Pacific Chinook salmon.

A thousand questions are raised against the genetically modified salmon. Most of these are on account of its effects on the environment. If the fish escapes from the hatchery, it can destroy the whole environmental balance because of its features that are claimed to be its advantages. It grows twice faster than the unmodified salmon, leading to an increased number of genetically modified salmon among the wild ones. The genetically modified salmons are able to survive longer than the wild ones. They switch their food they feed on, and adapt to any food source that is available for them. This feature of feeding which is more effective than usual, when combined with its capabilities to grow faster enables the genetically modified salmon to attain maturity and reproduce within a time span as low as two years. As the modified salmons achieve maturity faster, they will undergo smoltification also faster than the other fishes, which enables them to migrate to the freshwaters earlier. This also results in reduced competition for the modified salmons in foraging. If a complete depletion of food sources occurs, then they may feed on the wild unmodified salmons too. This will probably lead to near extinction of the wild salmons. Balance in food chain will be further disturbed as the genetically modified versions are capable of escaping from other living beings, which depend on salmons for their food, better than the other salmons. Other questions are regarding the safety issues on the consumption of genetically modified salmon. Many believed that it may contain many harmful substances that will affect the health of those who consume it more often. Even though tests have proved that there are no health problems due to its consumption, at least for a short term, some are still having the opinion that it will create problems on the long term consumption.

On account of the issues raised against the genetically modified salmon, the verdict of the Food and Drug Administration was eagerly awaited. The FDA stated that the genetically modified salmons will not cause any threat to environment and it’s completely safe as the Atlantic salmon. Those supporting and opposing this development are putting forward their own statements to prove their respective sides. The genetically modified salmons haven’t reached anyone’s dining table yet, but current FDA’s reports mean it will soon, as the first of its kind in the commercial market. To counter all the allegations against it, several explanations and remedies are being given by Aqua Bounty itself. They say that all the fishes produced will be sterile female and no male population will be produced, and hence there is no fear of multiplication of these fishes in the outer environment even if it escapes. Even in this context, issues are raised saying that 100% sterilization of the fishes cannot be achieved, and at least 5% of the total fishes produced will be fertile. Aqua Bounty says that farmers are given eggs of the fishes only, rather than providing them with young ones. Environmental activists are shutting their ears to all the remedies and explanations for the issues, and are strongly advocating that the genetically modified salmon can never be harmless to environment or humans. They add that tests conducted until now are only short term tests that cannot demonstrate the long term effects of it. The newly developed salmon is said to contain allergens that will cause severe health problems on continuous consumption of the fish. This problem has not received any explanation other than mere statements that the transgenic fish does not contain any harmful allergen. Tests also report that it does not contain any allergens, but still not giving a complete clarity for the issue.

From the first transgenic salmon that was made in 1989, the researches went at full swing, giving sleepless nights to scientists to reach the current milestone of producing a genetically modified salmon that can be applied on a commercial level. If the FDA further approves the production of the transgenic fish, it will soon reach the commercial market. The environmental activists are strongly opposing the whole development of transgenic fishes. The allegations against the transgenic salmon seem to be in need of genuine clarification to the public at least, as it is them who decide the fate of this fish as a commercial product. There is also a strong opinion to label the transgenic salmon, rather than selling it in the name of common Atlantic salmon, so that people can be selective about what they need. But here also FDA has taken a stand in support of it, claiming that as the transgenic salmon is completely safe to consume, so there is no need of any such labels that differentiates genetically modified salmon from natural salmon and consumers are required to do a wild guess here.

Putting aside the issues aside, on the assumption that all of those are solved and found a permanent solution, the genetically modified salmon is a big leap forward in the commercial aquaculture. It can provide food security up to a great level as the fishes can be produced twice as fast, as well as twice the weight of a natural salmon.

Common Challenges of Bioreactors

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Bioreactors are containers with a life supporting environment within them, used to carry out biochemical reactions using living organisms or chemical derived from living organisms. Algal bioreactors are those based on photosynthesis utilizing green algae for various purposes such as biomass production, CO2 fixation, etc. It has attracted much of world’s attention due its capability of producing bio diesel and bio ethanol.

In the current scenario, where fuel prices are going up fast, bio diesel and bio ethanol are inviting more use as additives to fossil fuel or even as itself. These demand mass production of both the mentioned bio fuels. Here arise the challenges faced in setting up and maintenance of bioreactors. Right from the maintenance of the bioreactor environment which includes irradiance levels, CO2 concentration, pH, salinity, nutrient levels, O2 concentrations etc., to unresolved issues affecting the overall efficiency of the bioreactor. Efficiency is crucial for any bioreactor. Any critical change in the internal environment of a bioreactor will end up in its reduced efficiency.

Challenges related to a bioreactor start right from its construction and setting up. A location for it needs to be chosen wisely. In the construction and setting up of bioreactors, geographical factors play a vital role. Moisture of the selected area seems to be a challenging task, taking into account, the levels of optimum moisture needed. For example, the average moisture levels in northwest countries are much higher than the required optimum moisture levels of an algal bioreactor, whereas in dry desert zones it is way too low than the required. Any faults during the operational procedures hence may lead to serious drops in the efficiency of the bioreactor, or even total loss of the whole contents of the system in extreme cases. Problems related to sterilization could be pointed out here, in the case of complete loss of the bioreactor contents. Around 3-5% of bioreactors set up every year are being failures due to the problem of sterilization. Hence, an aseptic operation is strictly required for a bioreactor. Single use bioreactors gained much attraction from the fact that it skips dangers of sterilization problems. Single use bioreactors are made up of disposable bags rather than from glass or steel as in conventional bioreactors. The disposable bags used are sterilized, minimizing further sterilization requirements. Also when at a risk of breach of sterilization standards massive loss can be prevented. Single use bioreactors are said to heavily cut down costs of setting up a bioreactor. Cost of setting up a bioreactor is challenge faced while constructing one. Until now, the costs of mass production bioreactors meant for large scale production of those like bio fuels, are not at all as low as to extract all the positives of a bioreactor. Single use bioreactors may cut down the cost of setting up by around 60%, as its components are only disposable bags consisting of three layers. But maximum capacities of these single use bioreactors are only around 1000 liters, or up to highest of 2000 liters, which can’t be used when much higher capacities are needed. Also, the single use bioreactors show its disadvantage in the achievable oxygen transfer rate, which sets the advantage of costs upside down, and limiting the single use bioreactors to the pharmaceutical field mostly.

When the bioreactor is ready to start working, crucial challenges comes up. Most important aspect that needs to be taken care is the control of the biochemical reaction environment, which is the key factor that aids in the maximum productivity. Temperature is another major factor just like the moisture. Hence, for this purpose the heat load should be calculated, equating heat production rate, reactor liquid volume, specific growth rate, biomass concentration, and yield coefficient. Then heat transfer methods can be employed accordingly. Heat transfer can be done externally or internally. For large reactors internal methods should be used because only internal methods can soak up heat in larger quantities compared to external methods. While smaller reactors require only external cooling jackets and external cooling coils, which have got the advantage of being free from cleaning problems. Internal heat transfer methods are difficult to clean, and they are easily fouled by the cell growth on the surface. Another challenge to overcome is the gas transfer, which can be accomplished through agitation. Agitation can be caused by mechanical stirring or can be air driven agitation. Mechanical agitation is possible only for small reactors, viscous liquids or those with low heat reactions. Bioreactors and those with high reaction heat can depend only on air driven agitations. In the ongoing maintenance of a bioreactor the foam that accumulates at the top needs to be removed. Mechanical foam breakers or chemical anti foam agents are used to remove the foam and prevent foam formation respectively. In the case of aerobic bioreactors, providing adequate aeration is task that needs proper attention, otherwise seriously causing steep decline in productivity and efficiency of the bioreactor. Anaerobic bioreactors are devoid of this problem as its efficiency does not depend upon aeration. By obtaining the accurate leachate data, most of the key challenges in the internal environment control of a bioreactor can be accomplished. Also, when air inputs are given for aeration purposes in an aerobic bioreactor or for the agitation purposes for heavy mass and high reaction heat bioreactors, dry air inputs needed to be given. Then evaporation needs to be taken care of with appropriate methods.

Material used for the construction of the bioreactors is critical area of attention. Only material which is completely anti corrosive and could be used for construction is glass. But it can only be used for construction of small bioreactors. Larger bioreactors need metal itself for the construction purposes. On long term use of bioreactors, the main problem encountered is the corrosion of the metal area of the bioreactor. The metal parts will be in a hostile environment due to the varying pH levels and salinity of the contents in the bioreactor. The metal surfaces need to be coated with anti corrosive substances, for the prevention of damage caused by corrosion. For this purpose mostly iron mica containing anti corrosion solutions are used.

The all these issues need to be resolved to set up and run a bioreactor at its perfection. And, the extra researches in overcoming them are worth the effort because of the numerable applications of bioreactor, including those we are having now, and those we are looking to the future.

Preserve Species by Changing their Genetic Structure

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A study on how the scientists are trying to preserve species by altering their Genetic Structure.

In the Mojave Desert in the U.S. state of Nevada, a few hundred meters above the famous Valley of Death, in a small underwater, probably the most endangered species of fish in the world has found its home. It is a silver-blue fish smaller than your smallest finger which came after millions of years of evolution to the edge of extinction. By unusual set of circumstances Cyprinodon species Diabolis (The Devils Hole Pupfish)is today reduced to only 75 living specimens.

The Cause of The Problem

Interestingly, the greatest threat to the survival of this species comes from a rather unexpected place - its DNA. In the past, these fish lived in a relatively large lake. About 20,000 years ago, the water level in the lake dropped very quickly, the landscape began to resemble the desert and the unfortunate fish found itself trapped in small physically separated lakes. So now there are nine different types of this fish, of which at least half are at the edge of extinction. The hardest case is the type of Cyprinodon Diabolis. Its population in September this year dropped to only 75 living members. Thousands of years of evolution made this fish capable of living in a very specific environment, to survive she needs water heated at thirty degrees Celsius, low oxygen saturation and a shallow part of the lake into which they retreat in the spawning season. Being an endangered species is already difficult enough, but to be an endangered species and at the same time picky regarding conditions in the environment is a totally new level of threat.

The Bad Luck

But that's not all. Besides being compromised and picky, this type of fish is followed by bad luck. In the early seventies of the last century American biologists have built three artificial lakes in which they tried to grow the backup fish from Devil's Cave (the location where the fish are located), to save them from collapse. Valves and other mechanical components cluttered up almost daily, and most of the fish died. When we say that this is a bad luck, then we really mean it, after completed repairs remaining fish were killed by a lightning strike that destroyed a generator for electricity, and that allowed the maintenance of very specific conditions in which this little fish could survive.

However, luck smiled to the fish that were in the third lake named Point of Rocks. Specifically, in this isolated population has somehow got the other related species of small fish and its DNA soon spread through the population like a wildfire. After about 50 years, every fish that was in the lake was a descendant of "intruders" who is who knows by what combination of circumstances made it into the lake. Scientists are able to very easily conclude this, as the hybrids between the two species had one pair of gills more than original type which they had tried to preserve. After some time, biologists who worked on the project, decided to move the fish into the hatchery. Unlike Cyprinodon Diabolis, which this facility led to the edge of extinction, a new hybrid species has simply exploded. "Huge aquariums we had were filled with new fish in very short time from top to bottom. Just a few decades ago, this species was dying, and now it suddenly experiences an unexpected increase of the population, "says Andy Martin, an evolutionary biologist at the University of Colorado, head of the group of scientists who investigated the specificity of DNA in a new hybrid species.

For Martin, this fact was clear evidence of what went wrong with the species Cyprinodon Diabolis – It was a genetic mutation. On the positive side, now that the diagnosis is finally established, there is a hope is that it is possible to find a drug that could save this fish. Martin claims to have a plan to rescue "the devil fish” from collapse, but for all those people who have in the past engaged in saving the various species from extinction, this idea probably will sound like a heresy tough.

The New Logic

For many years, scientists who were trying to preserve plant and animal species kept the same simple logic: it is necessary to protect the species X, as it existed at the time Y, in the place Z. Of course, evolution does not know the logic of this type, and changing is the only thing we can expect in all living beings on the planet. And here we come to Martin's heretical idea - he thinks that, in this case, the solution is not the preservation of species X as it was at the point Z, but the "breeding" of X species genes which would enable it to do what the evolution didn’t – the change. In other words, Martin wants to enrich this endangered species with the genes of her cousins - the type Cyprinodon nevadensis. those species have the common ancestor, and, interestingly, they live in small lakes just a few kilometers away. Martin's idea is pretty simple - throw a few individuals Cyprinodon nevadensis in the lake with the remaining 75 Diabolis and - wait. These few intruders would probably allow endangered species to stop spreading defective genes, says Martin. By the introduction of new genetic material this species could be saved, but the question is whether the individuals that survive could still be considered the same species.

This way, the small fish from the Mojave Desert have made us think about the one originally philosophical question of our relationship to the nature. It is already clear that we are likely to encounter in the future a variety of other endangered species, and the way we treat the problem of fish from Devil's Valley could become a precedent for all future cases. Scientists involved in the preservation of animal species therefore could become architects of the completely new ecosystem. Approach they have used until now was restricted to the exclusion of individuals from the wild and maintaining them in controlled and less risky conditions. But with this new approach, scientists will by manipulating the genome, practically create new species, which will contain smaller or larger fragments of those species they have tried to preserve. The idea is definitely controversial and Martin currently doesn’t have the support of the institution he is employed in. However, around the world there are a lot of endangered species and some are already preserved from extinction in a manner similar to that advocated by Martin.

References:
Written by Sasa (sale0303)

Isolation of the Gene Sequence of Interest from the DNA Libraries

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Knowledge on the complete genome of different organisms paves way for many developments and inventions in the field of molecular biology contributing to the advancement in various fields like medicine, Bio technology, Scientific research in the development of new medicines, new biotechnology products, understanding the genetic diseases and finding a cure for it through gene therapy, production of beneficial enzymes in large scale and so on. But there is always procedural difficulty to study the DNA of interest of any organism each and every time when required for the research. Hence molecular biologists found a solution to this problem by constructing gene library which holds the collection of DNA sequences derived from various organisms. They constructed two libraries, a genomic library and cDNA library.

A genomic library is created by extracting all the DNA from the nucleus of the selected organism, fragmenting the extracted DNA into suitable sizes using restriction enzyme, ligating the fragmented DNA into suitable vectors and introducing the vector with the inserted DNA sequence into suitable host bacteria. The group of transformed bacterial cells holds all the DNA sequences of the selected organism and is stored. A cDNA library is constructed in the similar way except that instead of the whole genome of the organism here the mRNA of the organism is turned into cDNA with the help of the reverse transcriptase enzyme which is then subjected to fragmentation and ligated into the vector and then introduced into the host bacterium. A genomic library holds the entire DNA sequences of an organism whereas the cDNA library holds the expression gene sequence of the organism as it is generated from the mRNA of the organism.

In this way molecular biologists construct the libraries and keep it ready so that as and when they want they can retrieve the gene of interest from the library for their research use. But extracting that particular DNA sequence or gene of interest from the whole lot of DNA sequences present in the library is quite a challenging task which is overcome by the development and application of various screening procedures and screening a genomic library and cDNA library have specific protocols. Hybridization technique, use of specific antibodies, hybrid arrest- hybrid release method, chromosome walking are some of the procedures applied to screen a gene library or cDNA library in order to isolate the gene sequence of interest.

Hybridization method employs the use of nucleic acid probes which are DNA sequences synthesized complementary to the gene sequence of interest to be extracted from the gene bank. The transformed bacteria carrying the vectors with inserted DNA sequence will either form colonies or plaques based on the type of vector used and the hybridization technique is also named accordingly as colony hybridization or plaque hybridization. Except for the plating procedure for the colony formation or the plaque formation rest of the procedure is similar. The group of colonies of transformed bacteria on a plate is imposed on a nitro cellulose membrane by placing the membrane on the plate. The membrane with copies of the colonies on the plate is then removed and the colonies on the plate are allowed to grow. The nitrocellulose membrane is then treated with sodium dodecyl sulfate and the protease enzyme inorder to lyse the bacterial colonies so that the DNA sequence gets exposed and adheres to the nitro cellulose membrane. The adhered DNA is denatured by treating with alkali like sodium hydroxide and the membrane is dipped in radioactive labeled nucleic acid probe containing solution. The nucleic acid probe attaches to the complementary DNA sequence on the membrane (hybridization) and the excess probe on the membrane is removed by washing. The areas of hybridization are seen by exposing the membrane to X-rays which is observed as dark spots on the X-ray film. The unique colony holding the gene sequence of interest is identified by comparing the main plate with bacterial colonies, the nitro cellulose membrane and the X-ray film. The identified colony carrying gene sequence of interest can then be extracted from the plate and grown as a step to amplify the gene of interest.

Using expression vectors in creating cDNA library makes the screening of the cDNA library much easier which is done with the help of specific antibodies. The ligation of cDNA fragments with special expression vectors enhances the transcription and translation of the cDNA sequence inside the host bacteria which in turn produces the protein coded by the cDNA. The screening procedure is similar to hybridization method except that here the nitrocellulose membrane holds the protein which is identified by using specific antibody which in turn helps identifying the particular bacterial colony producing the same protein of interest.

Thus screening procedures are the best index to find the gene sequence of interest from the constructed gene libraries.
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