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Biotechnology Forums - https://www.biotechnologyforums.com
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  • 08/13/18--12:41: Need a job
  • Hi Sir/Ma'am,

    I am fresher, I have completed my B. Sc +M.Sc in biotechnology in may 2018 only. I am not preparing for CSIR right now. I need a job related to my field only as my core interest is in R&D only. I have good internship records. Please suggest me some jobs suitable to my profile. I am very interested in Forensic DNA Analyst profile
    .
    With Regards.

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  • 08/19/18--07:41: Gate biotech preparation
  • I want to go for an offline coaching in pune for gate.Are there any please ppl let me know fast ?

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  • 08/19/18--08:16: Biotechnology
  • What is the scope and best institutes for pursuing msc in biotechnology if a student has PCB in class 12?

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  • 08/20/18--15:06: CRISPR Research Guidance
  • Hello, I'm posting here in hopes someone on this forum can point me in the right direction.
    I'm interested in genetically modifying avian species such as chickens. I am trying to make a breed of chicken that can have the attribute of a homing pigeon that allows it to find its way home.
    Does anyone have any books, threads, blogs, articles that someone with little knowledge about this topic can understand? Any help would be appreciated

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    Hi guys, how can one storage genetic material such as DNA at home for several years? 
    Also, what requirements should there be to the freezer that is used, for example should the 
    freezer be able to freeze at -20? 

    Thank you for answering.

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  • 09/01/18--00:09: Online courses
  • I m doing b tech biotechnology what are online courses I can apply for

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  • 09/01/18--11:22: Energy rich metabolites
  • Plzzz tell me abt energy rich metabolites

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    RECOMBINANT DNA TECHNOLOGY: OVERVIEW AND SCOPE

     
    SUMMARY
    Biotechnology is the application of technologies to the conventional biology with a goal of betterment of human life. The scope of biotechnology is vast and understanding the basics of biotechnology has become essential for the life science students. One of the important areas of biotechnology is recombinant DNA technology. This technology is the essence of many useful strategies widely used in healthcare, medicine, and nutrition. This article gives the basic idea about the topic and discusses its scope.
     
    BASICS
    Recombinant DNA is the DNA which is formed by the combination of DNA from two or more sources. While studying the basics of this technology, one must understand some important terms and students should know about:
    ·        Genome: The genetic constitution of an organism
    ·        Clones: Genetically identical organisms
    ·        Cloning: Introduction of foreign DNA into the host cell
    ·        Vector: Carrier of the gene of interest (ex. Plasmid, Phage)
    ·        Marker: Gene for the identification of transformant cells
    ·        Insert: Gene of interest carried by the vector to the host cell
    ·      Recombinant DNA: DNA formed by joining the DNA from different origins, mostly used for a combination of vector and insert
    ·        Host: Recipient cell for foreign DNA
    ·        Copy number: Copy number of a vector denotes the number of copies it can produce within a cell
     
    Tools used for recombinant DNA technology are the molecules, mainly enzymes, used for the manipulation of DNA like cutting, joining, end modification etc. (1). Some are enlisted below.
     
    ·        Restriction enzymes:  Popularly known as “molecular scissors”
    ·        Ligase: Popularly known as “molecular glue”
    ·        Topoisomerase
    ·        End modification enzymes: Polynucleotide kinases, nucleases
    ·        Linkers, adapters, and connectors.
     
    The central dogma of recombinant DNA technology
    A.  Insert selection: Insert is typically the gene of interest which we desire to clone
    B.   The formation of a recombinant DNA: It involves the use of restriction enzymes as well as a ligase. It is the joining of an insert to a vector.
    C.   Cloning of this recombinant DNA into a host cell.
    D. Expression of the gene of interest
     
     
    APPLICATIONS
    The whole aim for gene transfer exercises is either
    A.  Phenotypic expression of a foreign gene in an organism as can be seen in case of crop improvement techniques
    B.   Production of a particular protein in bulk
    Ex. Heterologous protein production like the production of human insulin in bacteria
     
    This technology is widely used in healthcare industries, agriculture, clinical research etc. Apart from this, it is also used for gene therapies including various genetic disease.
     
    FUTURE
    While using this technology, expressing a foreign gene in a given host is not that easy. Obviously, the host cell has to make a lot of compromises. Research is going on to address this issue (2). Recombinant DNA technology is further being expanded as synthetic biology (3), which will be the main driving force for therapeutics, food and energy sector and a lot more industries.
     
    REFERENCES
    1. https://link.springer.com/chapter/10.100...7-2460-8_3
    2. Ceroni F, Algar R, Stan G, Ellis T et al (2018) Nature Methods, 15:387–393, https://doi.org/10.1038/nmeth.4635
    3. Katz L, Chen YY, Gonzalez R et al (2018) J Ind Microbiol Biotechnol, 45: 449. https://doi.org/10.1007/s10295-018-2056-y

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  • 09/15/18--20:12: Carrer..
  • How to get a job in biotechnology field?

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    Can anyone suggest about any summer internship for biotechnology around the world?

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  • 09/16/18--20:03: Project
  • I am a student of MSc biotechnology in Bangalore please tell me the project topics which can be finished in 2.5months and which companies offer project for students .

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  • 09/29/18--19:05: Introduction
  • Hiii i am a new in this forum......
    Plz help me to know a recent Biotechnological researches and best project to do in UG level....

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    Hello all,
    I'm a student doing my bachelor's in Biotechnology Engineering.
    Hope I can contribute and learn more from this platform.
    Thank You

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  • 10/23/18--11:30: Perl programming
  • Hello
    From where i can learn perl programming for bioinformatics??

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    Registration for the ISGCT 2018 open .

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  • 11/03/18--06:13: Hellooooo!
  • Hello dude,

    Good evening to all. I'm a newbie here. I would like to introduce myself with all of you guys. I hope everyone here is doing well so far.

    Thank you so much!

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    Keep in mind the federal shape of India, there might be additives of GST – valuable GST (CGST) and nation GST (SGST). each Centre and States will concurrently levy GST throughout the price chain. Tax may be levied on every supply of products and offerings. Centre might levy and acquire significant items and services Tax (CGST), and States would levy and gather the state goods and services Tax (SGST) on all transactions inside a nation. The enter tax credit of CGST might be to be had for discharging the CGST legal responsibility on the output at each level. Similarly, the credit of GST Software paid on inputs could be allowed for paying the SGST on output. No move utilization of credit could be accredited.

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  • 11/08/18--21:49: wholesale Diaper Tote Bags
  • Our History
    Located in Xiamen, Fujian Province. We specilized in producing mother and baby products more then 10years. Quality is our culture,We are sure that our high quality products and excellent services will attract more and more customers' appreciation.
    Our Factory
    There are more than one hundred employees in our company. We made the baby carrier ,diaper bag  with the most eco-friendly and the most innovative materials.
    Our Product
    Diaper bag ,baby carrier, changing mat and other baby accessories ect.
    Product Application
    Baby carrier for 0-3 years baby ,diaper bag for and Mom and Dad.
    Our Certificate
    Our factory are well managed to reach ISO9001 standards .Also we applied social audit such as BSCI, SEDEX, SGS. For our products, we have passed the certification of EN-71,CE ect.
    Production Equipment
    Cutting Machine,Flat Sewing Machine ,High Head Sewing Machine,Pattern Sewing Machine ect.
    Production Market
    Our products have good reputation in Europe, America ,UK, Canada and etc.
    Our Service
    OEM &ODM are welcomed,Quality-Oriented,Customer -Orientedwholesale Diaper Tote Bags
    website:http://www.ababyproduct.com/

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  • 11/08/18--21:50: best Portable Camping Stove
  • Our History
    Our company was established in 2009 years.
    Our Factory
    YONGKANG NORTHSTAR OUTDOOR APPLIANCE CO.,LTD.. Our company was established in 2009 years. We are professional manufacturer and exporter that is concerned with the design,development and production of gas torch, camping stove.Our company responds to market trends, emphasizes customer service and satisfaction, and relies on the latest advanced technology. All of our products comply with international quality standards ,approve CE certificate.High quality , fast delivery time, good price, outstanding customer service is our advantage.
    Company Culture
    We adhere to the “excellent quality, reasonable price, prompt delivery capacity, and exceptional service” goals for our company to become your ideal partner in mutually beneficial business transactions.
    Value Outlook
    Within our company, we realize the importance of the “win-win” nature of doing business between ourselves and our customers. Based on this perspective, we spare no efforts to achieve breakthroughs in technology and production. We are firmly committed to providing our customers with high-quality and low-cost products, and with a consistent and reliable supply source.
    Our Product
    Gas Torch, Camping Stove
    Product Application
    Gas Torch: Lighting BBQ;s and fires. Thawing frozen water pipes. Hobby and Craftwork,Model Making. Soldering pipe joints. Paint stripping. Welding .Cooking and Catering-Flameing , Caramelizing,Browning, Adding Color and texture to food, Blistering and Skinning, Finishing foods for presentation and adding drama when serving.  
    Camping Stove: Cooking, whip up a hot meal
    Our Certificate
    CE certificate,Patentbest Portable Camping Stove
    website:http://www.chinanorthstar.com/