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Biotechnology Forums - https://www.biotechnologyforums.com
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  • 09/01/18--11:22: Energy rich metabolites
  • Plzzz tell me abt energy rich metabolites

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    RECOMBINANT DNA TECHNOLOGY: OVERVIEW AND SCOPE

     
    SUMMARY
    Biotechnology is the application of technologies to the conventional biology with a goal of betterment of human life. The scope of biotechnology is vast and understanding the basics of biotechnology has become essential for the life science students. One of the important areas of biotechnology is recombinant DNA technology. This technology is the essence of many useful strategies widely used in healthcare, medicine, and nutrition. This article gives the basic idea about the topic and discusses its scope.
     
    BASICS
    Recombinant DNA is the DNA which is formed by the combination of DNA from two or more sources. While studying the basics of this technology, one must understand some important terms and students should know about:
    ·        Genome: The genetic constitution of an organism
    ·        Clones: Genetically identical organisms
    ·        Cloning: Introduction of foreign DNA into the host cell
    ·        Vector: Carrier of the gene of interest (ex. Plasmid, Phage)
    ·        Marker: Gene for the identification of transformant cells
    ·        Insert: Gene of interest carried by the vector to the host cell
    ·      Recombinant DNA: DNA formed by joining the DNA from different origins, mostly used for a combination of vector and insert
    ·        Host: Recipient cell for foreign DNA
    ·        Copy number: Copy number of a vector denotes the number of copies it can produce within a cell
     
    Tools used for recombinant DNA technology are the molecules, mainly enzymes, used for the manipulation of DNA like cutting, joining, end modification etc. (1). Some are enlisted below.
     
    ·        Restriction enzymes:  Popularly known as “molecular scissors”
    ·        Ligase: Popularly known as “molecular glue”
    ·        Topoisomerase
    ·        End modification enzymes: Polynucleotide kinases, nucleases
    ·        Linkers, adapters, and connectors.
     
    The central dogma of recombinant DNA technology
    A.  Insert selection: Insert is typically the gene of interest which we desire to clone
    B.   The formation of a recombinant DNA: It involves the use of restriction enzymes as well as a ligase. It is the joining of an insert to a vector.
    C.   Cloning of this recombinant DNA into a host cell.
    D. Expression of the gene of interest
     
     
    APPLICATIONS
    The whole aim for gene transfer exercises is either
    A.  Phenotypic expression of a foreign gene in an organism as can be seen in case of crop improvement techniques
    B.   Production of a particular protein in bulk
    Ex. Heterologous protein production like the production of human insulin in bacteria
     
    This technology is widely used in healthcare industries, agriculture, clinical research etc. Apart from this, it is also used for gene therapies including various genetic disease.
     
    FUTURE
    While using this technology, expressing a foreign gene in a given host is not that easy. Obviously, the host cell has to make a lot of compromises. Research is going on to address this issue (2). Recombinant DNA technology is further being expanded as synthetic biology (3), which will be the main driving force for therapeutics, food and energy sector and a lot more industries.
     
    REFERENCES
    1. https://link.springer.com/chapter/10.100...7-2460-8_3
    2. Ceroni F, Algar R, Stan G, Ellis T et al (2018) Nature Methods, 15:387–393, https://doi.org/10.1038/nmeth.4635
    3. Katz L, Chen YY, Gonzalez R et al (2018) J Ind Microbiol Biotechnol, 45: 449. https://doi.org/10.1007/s10295-018-2056-y

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  • 09/15/18--20:12: Carrer..
  • How to get a job in biotechnology field?

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    Can anyone suggest about any summer internship for biotechnology around the world?

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  • 09/16/18--20:03: Project
  • I am a student of MSc biotechnology in Bangalore please tell me the project topics which can be finished in 2.5months and which companies offer project for students .

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  • 09/29/18--19:05: Introduction
  • Hiii i am a new in this forum......
    Plz help me to know a recent Biotechnological researches and best project to do in UG level....

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    Hello all,
    I'm a student doing my bachelor's in Biotechnology Engineering.
    Hope I can contribute and learn more from this platform.
    Thank You

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  • 10/23/18--11:30: Perl programming
  • Hello
    From where i can learn perl programming for bioinformatics??

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    Registration for the ISGCT 2018 open .

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  • 11/03/18--06:13: Hellooooo!
  • Hello dude,

    Good evening to all. I'm a newbie here. I would like to introduce myself with all of you guys. I hope everyone here is doing well so far.

    Thank you so much!

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  • 11/25/18--21:31: Introduction
  • Hello, everyone.i am gunaseelan ,2nd year btech biotech student.here many professionals guides biotech students .I am really happy to join this community.thanks everyone...

    Sent from my Redmi Note 5 using Tapatalk

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  • 08/19/18--08:16: Biotechnology
  • What is the scope and best institutes for pursuing msc in biotechnology if a student has PCB in class 12?

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  • 08/20/18--15:06: CRISPR Research Guidance
  • Hello, I'm posting here in hopes someone on this forum can point me in the right direction.
    I'm interested in genetically modifying avian species such as chickens. I am trying to make a breed of chicken that can have the attribute of a homing pigeon that allows it to find its way home.
    Does anyone have any books, threads, blogs, articles that someone with little knowledge about this topic can understand? Any help would be appreciated

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    Hi guys, how can one storage genetic material such as DNA at home for several years? 
    Also, what requirements should there be to the freezer that is used, for example should the 
    freezer be able to freeze at -20? 

    Thank you for answering.

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  • 09/01/18--00:09: Online courses
  • I m doing b tech biotechnology what are online courses I can apply for

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    Hi Everyone!
    I am really pleased to join your community. I find it really enriching and useful.

    Today I am writing to you with a really broad question regarding background referred to analytical chemistry.
    Currently, I am writing my Master thesis and due to the fact that am only an engineer of biotech; some from aspects of pure analytical chemistry is really not understandable.

    Honestly, I am left without an advisor and have nobody to ask for help.
    Lets move to the point; I am ordering my results and found myself in a position where I do not understand the difference in the application of cyclic voltammetry and square wave voltammetry.

    I made few measurements of a suspension containing an enzyme and a buffer ( not activity one ). I hold in a hand a publication where research group applied a square wave voltammetry and as a standardized value; applied a specific pick on exact potential. I will be really grateful for receiving a short explanation about the difference between those two techniques.

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  • 02/26/19--10:01: Seminar topics
  • Can anybody please suggest me some good seminar topics for biotechnology engineering

    Sent from my A1601 using Tapatalk

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    Is it possible to embed transmembrane domain to the protein by inserting sequence which codes mentioned domain to gene that codes the enzyme, in the way that it would allow the protein to become an IMP (integral membrane protein) without depriving it of its enzymatic properties?

    I would really appreciate a helpful answer.

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  • 08/19/18--07:41: Gate biotech preparation
  • I want to go for an offline coaching in pune for gate.Are there any please ppl let me know fast ?